Structure and Synthesis of a Lipid-Containing Bacteriophage. An Endolysin Activity Associated with Bacteriophage PM2
نویسندگان
چکیده
منابع مشابه
Inactivation of the lipid-containing bacteriophage PM2 by butylate hydroxytoluene.
Several factors have been investigated which are of significance in the inactivation of PM2, a lipid-containing bacterial virus, by butylated hydroxytoluene (BHT). Studies of the time dependence of inactivation during exposure to BHT showed that virus killing occurs rapidly, with the majority of the effect taking place in the first 5 min. The degree of inactivation is dependent upon the initial...
متن کاملGenome characterization of lipid-containing marine bacteriophage PM2 by transposon insertion mutagenesis.
Bacteriophage PM2 presently is the only member of the Corticoviridae family. The virion consists of a protein-rich lipid vesicle, which is surrounded by an icosahedral protein capsid. The lipid vesicle encloses a supercoiled circular double-stranded DNA genome of 10,079 bp. PM2 belongs to the marine phage community and is known to infect two gram-negative Pseudoalteromonas species. In this stud...
متن کاملPreliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2.
PM2 (Corticoviridae) is a dsDNA bacteriophage which contains a lipid membrane beneath its icosahedral capsid. In this respect it resembles bacteriophage PRD1 (Tectiviridae), although it is not known whether the similarity extends to the detailed molecular architecture of the virus, for instance the fold of the major coat protein P2. Structural analysis of PM2 has been initiated and virus-derive...
متن کاملMARINE ICOSAHEDRAL MEMBRANE-CONTAINING dsDNA BACTERIOPHAGE PM2: VIRION STRUCTURE AND HOST CELL PENETRATION
Original publications This thesis is based on the following articles, which are referred to in the text by their Roman numerals. Purification and protein composition of PM2, the first lipid-containing bacterial virus to be isolated. Virology 262, 364-374. PM2 has a protein capsid surrounding a spherical proteinaceous lipid core. The PM2 virion has a novel organization with an internal membrane ...
متن کاملA bacteriophage endolysin that eliminates intracellular streptococci
PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PlyC is a potent agent for controlling intracellular Spy that often underlies refractory infections. We show that the PlyC holoenzyme, mediated by its PlyCB subunit, crosses epithelial cell membranes and clears intracellular Spy in a dose-dependent manner. Quantitative studies using...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1977
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1977.tb11702.x